Aman Tedasen is a PhD student at Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Thailand. He received his Medical technology training and earned his Bachelor degree in 2012. He received a scholarship from the Royal Golden Jubilee PhD Program of Thai government. His research interest has focused on developing effective treatment strategies from herbal medicine for breast cancer.
5′-desmethoxyyatein, a natural lignan, has shown potent cytotoxic activity in many cancer cell lines including melanoma, leukemia, breast, colon and brain cancers. Molecular docking is a powerful computational tool to predict the binding mode and binding affinity of a ligand with the specific proteins. In this study, we aimed to investigate molecular targets of 5′-desmethoxyyatein from 80 proteins, which relate to cancer cell proliferation, cell cycle progression, apoptosis, or cell migration. Binding affinity evaluation of 5′-desmethoxyyatein to these proteins was carried out using Molecular docking method. Results showed that 5′-desmethoxyyatein may inhibit activin receptor 2 (ACTVR2), prostaglandin G/H synthase 2, human epidermal growth factor receptor 2 (HER-2), janus kinase 3 (JAK3), protein kinase C (PKC), heat shock protein 90-beta (Hsp90-beta), transforming growth factor receptor I (TGF-β receptor I), androgen receptor and NF-kappa-B-inducing kinase (NIK) proteins. This information was derived from better predicted binding affinity of 5′-desmethoxyyatein, compared to its known inhibitors. Moreover, 5′-desmethoxyyatein may hinder cell cycle progression via binding to cyclin A, polo-like kinase 1 (PLK1) and aurora A proteins with binding energy and inhibition constant lower than its known inhibitors. X-linked inhibitor of apoptosis protein (XAIP) and glycogen synthase kinase 3 (GSK-3β) proteins, involved in apoptosis and migration in cancer cells, also may better interact with 5′-desmethoxyyatein than their inhibitors. This study suggests 5′-desmethoxyyatein would be anti-tumor agent with anti-proliferation, cell cycle arrest inducing, apoptosis induction and anti-migration activities. However, further studies such as Western blot analysis are needed.
Chiayi Chou earned her MD degree from China Medica University in Taiwan in 2008. She specialized in acupuncture. After 3 years residency training in Tzu Chi Medical Center, she has experience in treating patients with various diseases, including cardiopulmonary diseases and sports, injuries rehabilitation and recovery. She has great passion and interests in sports medicine. She would like to utilize and apply acupuncture in exercise science. She is pursuing her MS degree in exercise science at Department of Kinesiology in University of Wisconsin-Madison.
Transcutaneous Electrical Acupoint Stimulation (TEAS) is a safe standardized non-invasive therapeutic technique to enhance the heart rate regulation in healthy and cardiovascular disease individuals PURPOSE: The aim of this study was to assess the acute therapeutic effect of TEAS on recovery heart rate (HR) after exercise. METHODS: Forty-one recreationally active college students were recruited and randomly assigned into either TEAS group (TG) or control group (CG). All participants were required to perform two trials of 3-minute step exercise (3MST) wtih a break of 30 minutes between trials . For the TG, participants received 10 minutes TEAS treatment on both forearms at the two selected standard acupoints (Nei-Guan [PC6] and Lie-Que [LU7]) for cardiovascular disease before the second trial, while the CG was seated at rest. HR was measured at rest (HRrest), the first (HR1), second (HR2) and third-minute (HR3) of recovery after 3MST. Mixed-models repeated measures method was used to compare differences between the groups (α = 0.05). RESULTS: There were no significant difference in HRrest1 and HRrest2 between the first and the second 3MST in both groups, as well as between groups. Groups HRrest1 (beats/min) HRrest2 (beats/min) 3MST Fitness Index (Pre-treatment) 3MST Fitness Index (Post treatment) TG 81 ± 7 82 ± 8 56.93 ± 8.43 58.31 ± 11.80 CG 81 ± 10 82 ± 11 58.31 ± 11.80 58.13 ± 6.09 The caluculated 3MST fitness indexes were no significance different between the Pre-treatment and the post treatment in both groups. CONCLUSION: Although TEAS at PC6 and LU7 has demonstrated it can slowdown the heart rate in tachycardia patients. But in this study, it did not show the effect. We spaculate, the timing and durantion of TEAS treatment may play important role and its machanism on enhencing cardiovascular enhencing need to further investigate. Keywords: theraputics, cardiovascular, regulation, fitness