Traditional & Alternative Medicine

Breast cancer is one of the most prevalent diseases and a major cause of death in women around the world. Th e rind of asam kandis (Garcinia cowa Roxb.) contains xanthone which has potential as anticancer. In previous study, DCM fraction of the rind of asam kandis showed cytotoxic eff ect on T47D breast cancer cells and also potentially inducing cell death (apoptosis) of the cancer. Th is study observes how the level of TNF-α is aff ected by the fraction from the rind of asam kandis in T47D breast cancer cells. TNF-α is known as an inducer of the death (apoptosis) in cells generally and as survival in tumor cell line. Levels of TNF-α are known by using ELISA method based on the principle reaction binding between antigen and antibody. Th e result showed that the average levels of TNF-α in each of control and treatment (0.1; 1.10 and 100μg/mL) were not signifi cantly aff ect the levels of TNF-α with P value > 0.05.