Traditional & Alternative Medicine

An in vivo study of the activity of scopoletin isolated from noni fruit (Morinda citrifolia L.) on the level of interleukin-4 (IL-4), IL-10 and IgE in type I hypersensitive male Swiss-Webster mice has been carried out. Scopoletin was isolated from dried noni powder by soxhletation method using dichloromethane, separated by column chromatography using silica gel as stationary phase and n-hexane-ethyl acetate (1:4) as mobile phase, then purifi ed by column chromatography using Sephadex LH20 as stationary phase and methanol as mobile phase. Type I hypersensitive male mice were obtained by ovalbumin sensitization. Animal model were divided into 5 groups: negative control group, positive control group, and scopoletin-treated group (1; 3; and 10 mg/kg). Th e results showed that scopoletin at doses of 1, 3 and 10 mg/kg decreased the level of IL-4 of type I hypersensitive mice signifi cantly (p<0.01). Th e scopoletin at the dose of 10 mg/kg decreased the serum level of IL-4, IL-10, and IgE (P<0.01) to the normal level. Th e ability of scopoletin to decrease IL-4 and IgE concentration of type I hypersensitive mice to its normal state was shown by dose of 10 mg/kg BW (p>0.05), while for IL-10 concentration, the decrease until its normal level was shown by dose of 3 mg/kg BW (p<0.05).