Traditional & Alternative Medicine

Breast cancer is one cancer with the highest of frequency occurrence among other types of cancer in women. Meanwhile anticancer drugs that exist today do not provide optimum results in therapy because less selectivity. Th erefore, there is a needed to fi nd new drug source from natural origin. One of the eff orts was to evaluate cytotoxic activity in vitro of cowanin compound from the bark of Asam kandis (Garcinia cowa Roxb) against T47D breast cancer cells. Th e potency of cowanin were tested using MTT method, a colorimetric assay based on capacity of mitochondria succinate dehydrogenase enzymes in living cells to reduce MTT salt into an insoluble, colored formazan product which was measured spectrophotometrically. Cowanin compound made into a solution of the test preparation in four concentrations is 0.1μg/mL, 1 μg/mL, 10μg/mL, and 100μg/mL. Testing has been done giving IC50 values cowanin compounds against T47D breast cancer cells amounted to 6.986±0.786μg/mL. Statistical analysis showed that the compound cowanin of bark of asam kandis can inhibit the growth of breast cancer cells T47D signifi cantly at a concentration of 100μg/mL

(P<0.05).